How to Run A/B Tests on Email Subject Lines in Letterhead

This article explains how to set up and run an A/B test on email subject lines in your newsletters. A/B testing helps you automatically send the best-performing subject line to most of your audience, boosting open rates and engagement.

Before You Start

  • You’ll need at least 400 audience members for A/B testing to work.

  • A/B testing is currently available only for email subject lines (not preview text or newsletter content).

  • The feature automatically splits your audience:

    • 25% receives the test (split between two subject lines).

    • 75% receives the winning subject line based on open rates.


Step-by-Step Instructions

  1. Go to your Newsletter draft.

    • From the left-hand menu, select Newsletters.

    • Choose the draft you’d like to edit.

  2. Proceed to the Preview & Schedule step.

    • Click Continue editing until you reach Step 2: Preview and Schedule.

  3. Enable A/B Testing.

    • Toggle the switch next to Create A/B Test.

  4. Enter your subject lines.

    • In the Email Subject fields, type two different subject line variations you want to test.

  5. Set your send details.

    • Choose your Audience.

    • Pick your Date and Time to send.

    • (Optional) Send a test email before scheduling.

  6. Schedule your newsletter.

    • Click Schedule.

    • Letterhead will send the subject line variations to 25% of your audience, measure open rates, and automatically send the winning subject line to the remaining 75%.


Reviewing Results

After your newsletter is sent, you can view the results in the Newsletter Metrics dashboard:

  • See both subject lines with full text.

  • A “winner” badge will mark the best performer.

  • Metrics include:

    • Opens (count)

    • Open Rate (%)

    • Recipients reached


Troubleshooting / FAQs

  • What happens if my audience is smaller than 400 members?
    A/B testing requires at least 400 recipients. If your audience is smaller, the toggle won’t be available.

  • Can I test more than two subject lines?
    Not at this time. Each test supports two subject line variations.

  • Where can I see past results?
    Go to your Published Newsletter metrics. Each newsletter will display the A/B Test Results section if it includes a test.


    Need Help?

    If you have questions or need assistance, please contact our support team at support@tryletterhead.com or log a support ticket through the Help Center!